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CONTENTS YEAR 2012 Asian J. Exp. Sci., V 26, No. 2, 2012; 01-04
Control of Propionibacterium acnes using Essential oil
Smruti Thombare and Usha Mukundan
Department of Botany, Ramniranjan Jhunjhunwala College, Ghatkhopar, Mumbai (Maharashtra), India. Email: smrutithombare@yahoo.com
Abstract : In the present investigation the antimicrobial effect of 19 plant essential oils have been studied on Propionibacterium acnes, the bacterium causes human skin disease, the Acne vulgaris. Out of 19 essential oil, 5 oils showed inhibitory effect against this bacterium. These plant oils used are from Thymus vulgaris (thyme), Eucalyptus globulus (eucalyptus), Syzygium aromaticum (clove bud), Cinnamomum verum (cinnamon) and Melaleuca alternifolia (tea tree), they have shown effective inhibition zone in mm as 29.66±0.5, 25.00±0.0, 55.33±0.5, 18.00±0.0, 13.66±0.5 respectively. The anti acne cream was prepared containing 2% of each of the effective essential oil, stearic acid (24.2%), cetyl alcohol(1.20%), triethanolamine (1.32%), propylene glycol(0.95%), isopropyl myristate (1.20%), glycerine (2.00%), borax (1.90%). Anti acne cream formulated showed the anti activity against P. acnes.
Key words : Propionibacterium acnes, Acne vulgaris, Minimum inhibitory concentration, Anti acne cream. chromate
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Asian J. Exp. Sci., V 26, No. 2, 2012; 05-10
Genomic Analysis of Bacterium Isolated from Amended Coal Fly Ash by 16S rDNA
Sitara Jabeen and M. P. Sinha*
Department of Zoology, Ranchi University, Abstract : Fly ash being the problematic solid waste must be managed in eco-friendly way. For the same the
present study was conducted to analyse the FA resistant bacteria by16S rDNA PCR. The identified bacterium was
Kocuria sp HO-9042 with Gen bank Accession No: DQ531634.2. PCR amplification of the 16S rDNA gene from
Kocuria sp revealed 8F and 1492R sequences with 550bp and 985bp respectively and a consensus sequence of
1401bp. The distribution of 100 BLAST hits on the 1401 query sequence showed the 10 homologs with about 200
nucleotide similarities with the homologues showing close relatedness on the basis of the sequence similarity in the
significant alignments. The present investigation suggests that Kocuria sp HO-9042 in coal fly ash amended soil
will also help in improving soil fertility.
Key words : Fly ash, 16S rDNA, Kocuria, PCR, BLAST. - - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - Study of Levels of Heavy Metal in Soil under Amravati Municipal Juridiction, Maharashtra (India) S. K. Gudadhe*, V. S. Manik, P.B. Deshbhratar and D. S. Ramteke National Environmental Engineering Research Institute (NEERI), Abstract : The study involves the atomic absorption analysis of six selected heavy metals lead, cadmium, zinc,
copper and chromium pollution in the soil sample of Amravati municipality, Maharashtra. A total of 160 surface soil
samples (0-5 cm.) and 160 sub-surface soil sample (10-15 cm.) were collected from industrial, agricultural, road
side and residential area. The result shows that the level of cadmium and copper exceeded the recommended level.
Lead and zinc pollution was found only in the industrial area cobalt pollution was found in the industrial and road
side area. There was no chromium pollution in the soil samples analyzed. Key words : Atomic Absorption Spectroscopy, Heavy metals, pH, Organic content - - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - Evaluation of Soil Microfauna under Parthenium hysterophorus (L.) Vegetation Priyanka Saha*, Bharti Singh Raipat* and M. P. Sinha** Department of Zoology, Ranchi University, Ranchi-834008 Abstract : Experiments were performed to evaluate the microfauna of soil under Parthenium hysterophorus
vegetation. Soil samples were collected from a depth of 10-15cm, at an interval of 15 days for two months from
dense vegetation of Parthenium and nearby normal vegetation from agro-ecosystem. Three types of bacterial
colony viz. circular-entire, irregular-undulate and punctiform-entire were observed in normal soil while in
Parthenium affected soil one additional filamentous type of colony was found. All types of colonies showed white
pigmentation and Gram positive reaction. Quantitatively the bacterial population of affected soil sample was
always significantly less than (p < 0.001) normal soil sample. Mean bacterial population of normal soil sample was
33.4±0.989×109, 52±0.866×109, 22.5 ±1.50×109 and 42.1 ±1.724×109 and in Parthenium affected soil it was 28.3
±1.921×109, 49.4 ±1.40×109, 16.4 ±2.138×109 and 34.6 ±1.040×109. A similar trend of significant decline in wet
weight (mg /g soil) and biomass (mg /g soil) of bacteria was observed between normal soil samples and affected
one. The results indicated that Parthenium hysterophorus imparts adverse effect on soil bacteria. The Gram positive
bacterial colony with punctiform-entire, shape and margin appeared to be resistant to Parthenium infestation in
comparisons to other bacteria. Hence, bacteria colony having such type of colony morphology can be used as
bioindicator for pollution caused by Parthenium. Key words : Parthenium hysterophorus, Microfauna, Parthenin, Bioindicator. - - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - Evidence for the Alteration of Plasma Calcium by Synthetic Glucocorticiod Dexamethasone
N. Venkat Appa Rao*, Rakhee Dubey and Balarko Chakraborty Department of Zoology, St. Xavier's College, Ranchi -834 001,India. Abstract : The present investigation deals with the effect of dexamethasone, a synthetic glucocorticoid on plasma
calcium concentration in guinea pigs. The administration of dexamethasone @ 2.5 mg/kg body weight for seven
consecutive days results to an elevation of plasma calcium concentration from a mean value of 4.51±0.106 mol eq/L
to a mean value of 5.85±0.102 mol eq/L, an increase of about 30% which is statistically significant. The probable
mode of action for dexamethasone is suggested. Key words : Dexamethasone, Guineapigs, Plasma calcium , Hypercalcemia. - - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - Community Based Disaster Management (CBDM) - A surveillance of Chhattisgarh. Arti Singh*, Raja Jaiswal ** *Assistant Professor, Abstract : The information about the disaster management program generated by the Government has not been
disseminated in the communities properly, particularly in tribal population. The present study suggests that
community disaster management education is essential and will develop capacity building amongst community in
general and tribal population in particular to face disaster. The government should focus on the implementation of
the programs at the grass root level through active community education. It is suggested that community level
mitigation programs (CLMP) should also include structural and non-structural measures. The structural measures
are safe construction, retrofitting, community shelters, legal framework while non-structural measures include
financial resources in which Government(s) should actively involve Insurance companies, Municipalities,
Corporations, Corporate houses Boards, Nigams , District Administration, Panchayats, Block level and Village
level committees for providing sources of obtaining funds. Utilization of funds and awareness of proper
construction of houses disaster free to avoid loses of the property and human life in any disaster. It is also suggested
that survey through satellite be made for locating fresh underground water resources on account of draught due to
short rain fall. Key words : Community, Disaster, Management, Technology, Information. - - - - - - - - - - - - - - - - - - - - - - - -
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Substantion of the Parameters to Tilt the Camera Advanced Cleaning Seeds Pasture Plants M.S.Toilybaev , Zh.S.Sadykov, Sh.A.Alpeisov, N.S.Toilybaev Kazakh National Agrarian University ,Abay prospect 8, Almaty, Kazakhstan Abstract : To improve performance, harvesting seed pasture plants we have developed and refined a promising
new generation of camera tilt grain-harvesting machines. To study the optimal parameters improved feeding
channel for harvesting seeds of pasture plants, in particular, methods of wheatgrass our experimental design, which
consists in choosing the number and the experimental conditions, necessary and sufficient for the task with the
required accuracy? Optimal parameters of the improved tilted camera have been discussed. Key words : Tilt of camera, Cleaning seeds, Pasture plant. - - - - - - - - - - - - - - - - - - - - - - - -
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Anita Kumari Gupta, and Hari Om Pandey 1. Assistant Prof., S.S. Memorial College, Ranchi. Abstract : Of late, ditertiary butyl chromate (TBC) and ditertiary amyl chromate (TAC) have attracted much
attention of chemists as chromium (VI) based oxidants for organic synthetic processes. This is due to their stability,
versatility, controllability of the reactions and, of course, due to better yield of the products. In the present work, we
have carried out the oxidation of propane-1,2-diol with TBC and TAC in different solvents like tetrahydrofuran ,
acetonitrile, dioxane, dichloromethane etc. The products, thus, formed were purified, tested for purity, analysed
spectroscopically and thermogravimetrically and formulated so as to make some generalization in regards to the
role of solvents in such redox processes. Key words : Propane-1,2-diol, Dioxane, Tetrahydrofuran, Acetonitrile, TBC, TAC. - - - - - - - - - - - - - - - - - - - - - - - -
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Report on Heteroceran Lepidoptera diversity of Harmu, Ranchi, Jharkhand Anand Kr. Thakur*, Nabonita Sarkar**, Arti Singh** & Nanda Ghosh** *Department of Zoology, S. S. Memorial College, Ranchi, Jharkhand Abstract : Heteroceran lepidoptera (moth) are common pests over plants and damage leaves, stems, flowers and
fruits. A study was carried in Harmu, Ranchi between January 2010 and December 2010. A total 11 species
belonging to 4 families (Tortricidae, Pyralididae, Noctuidae and Sphingidae) were collected. The statistical
interpretations were carried out by using Shannon-Wiener diversity index and Shannon's equitability. Biodiversity
of moth with host plants was observed and species richness was observed 2.37. The species richness and abundance
are indicative of that the Harmu area has good representation of moth diversity. Key words : Heteroceran lepidoptera, Shannon-Wiener diversity index, Harmu, Ranchi. - - - - - - - - - - - - - - - - - - - - - - - -
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A Report on Butterfly Diversity of Reclaimed OBDs of Kathara Coalmine Area, Jharkhand *Amita Hembrom and **Braj Kishore Sinha *Research Assosiate, Department of Zoology, S S M College, Ranchi University, Ranchi, **Asst. Professor, Department of Zoology, S S M College, Ranchi University, Ranchi Abstract : Butterflies are one of the largest groups of insects and are most noticeable due to their conspicuous
nature in a landscape. A study was carried out of four different aged reclaimed over burden dumps (ROBDs) of
Kathara coalmine area of Jharkhand from 2007 to early 2009 and the present paper is a report on the diversity of the
recorded butterflies. About 327 specimens of butterflies were collected from 10 randomized quadrates of 10 m x 10m along transact. Out of total observed butterflies twenty seven species belonged to four families: Pieridae,
Lycaenidae Nymphalidae and Papilionidae of the supper family Papilionoidea. The family Pieridae dominated the
ROBDs with their highest occurrence (44 per cent). It was observed that the newly ROBDs are having higher
diversity index than the older ROBDs. Key words : Butterfly, Reclaimed over burden dumps (ROBDs), Diversity index, Habitat fragmentation. - - - - - - - - - - - - - - - - - - - - - - - -
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Modulation of radiation induced oxidative damage in brain of Swiss albino mice by flaxseed oil. Shikha Patni*, Rashmi Sisodia* and Preeti Shrivastava** *Department of Zoology, University of Rajasthan, Jaipur, India Abstract : Study was done to evaluate the biochemical and histopathological changes after 5 Gy whole body
irradiation and its modulation by supplementation of flaxseed oil on male Swiss albino mice brain up to 30 days. For
this, healthy mice from an inbred colony were divided into four groups: (i) control (vehicle treated) (ii) flaxseed oil
treated : mice in this group were orally supplemented with flaxseed oil (100 Ul/mice/day) once daily for 15
consecutive days; (iii) irradiated mice (iv) FO + irradiated : mice in this group were orally supplemented with FO for
15 days (once a day) prior to irradiation. Marked radiation induced changes in the amount of whole brain lipid
peroxidation (LPO), Glutathione (GSH), protein, nucleic acids and histopathological changes could be significantly
(p < 0.001) ameliorated specially at later intervals by supplementation of FO prior to irradiation. The results of
present study showed that prior supplementation of FO has radioprotective potential as well as neuroprotective
properties against the radiation. Key words : Whole brain, Cerebellum, Oxidative stress, Lipid peroxidation, Glutathione, Nucleic acids, Purkinje cells. - - - - - - - - - - - - - - - - - - - - - - - -
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Neuronal morphology of dorsal cerebral cortex of the Indian wall lizard, H. flaviviridis (Rüppell). R. C. Maurya and U. C. Srivastava Department of Zoology Abstract : The cerebral hemisphere of lizards is divided into pallium (cerebral cortex) and subpallium. The
structural organization of dorsal cerebral cortex of the H. flaviviridis has been studied with the help of
neurohistological techniques. The dorsal cortex shows three neuronal layers viz. outer plexiform layer, middle
cellular layer and inner plexiform layer. Using characteristics such as criteria of location, soma shape and size,
dendritic tree pattern/dendritic field shape, and dendritic spine covering four types of neurons have been
distinguished in the cellular layer of dorsal cortex: monotufted neurons (18.14 %), bitufted neurons (28.43 %),
multipolar neurons (22.55 %) and pyramidal neurons (30.88 %). The comparative study of the morphology of these
neurons represents their similarities with other animal groups. Key words : lateral cortex /bitufted neuron/ spinous/Golgi study/lizards. - - - - - - - - - - - - - - - - - - - - - - - -
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