AJES
Asian Journal of Experimental Sciences
 
 
AJES

 

 


VOLUME 19: ISSUES 2

CONTENTS YEAR 2005

Click on the linked title to view the paper  

Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 1-12

Ecological Overshoot and Ecological Restoration

John Cairns, Jr.
Department of Biological Sciences,
Virginia Polytechnic Institute and State University,
Blacksburg, Virginia 24061, USA

Abstract: Persuasive evidence indicates that humankind inhabits a planet with an ecological overshoot, i.e., humankind is using ecological resources faster than they can be restored. In addition, human society is releasing pollutants and wastes more rapidly than natural systems can assimilate them. Ideally, the pollutants and wastes should be assimilated and transformed to a degree that they benefit other species. If overshoot persists, it could well result in a global environmental collapse that, in turn, would result in an economic collapse. To prevent such a disaster, human society must replace unsustainable practices with sustainable practices. This article focuses on ecological restoration, which increases natural capital (i.e., natural resources) and the ecosystem services it provides. Societal change with ecological restoration should reduce the overshoot and make sustainable use of the planet possible.

Key words: Ecological overshoot, Ecological restoration, Natural capital, Ecosystem services, Restoration, checklist, Sustainability.
 

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 13-22

Biodiesel as an Alternative Fuel for Pollution Control in Diesel Engine

MS. P. Chincholkar, Saurabh Srivastava, A. Rehman, Savita Dixit and Atul Lanjewar
Mech. Engneering Department and Applied Chemical Department
Maulana Azad National Institute of Technology,
Bhopal – 462007; India

Abstract : Diesel vehicles are the major source for air pollution; there is great potential for global warming due to discharge of greenhouse gases like CO2 from vehicles. Many lung problems are connected with particulate matter emitted by diesel vehicle including dust, soot and smoke. People are exposed to pollution even as they talk or when stir up the dust when they walk. Biodiesel is a non-toxic, biodegradable and renewable fuel. Compared to diesel fuel, biodiesel produces no sulfur, no net carbon dioxide, less carbon monoxide and more oxygen. More free oxygen leads to the complete combustion and reduced emission. Overall biodiesel emissions are very less compared to diesel fuel emissions which is promising pollution free environment.
Abundant source of vegetable oil in India and its ease of conversion to biodiesel help to save large expenditure done on import of petroleum products and economic growth of country. Bio-diesel also generate huge rural employment and degraded lands can be restored due to plantation of oil plants which helps in reducing pollution. Lot of work is going on in different countries on different types of vegetable oils like sunflower oil, karanji oil, linseed oil, soya bean oil, palm oil and many more, which can be used in those countries as per availability, our research is in progress on jatropha oil, jatropha oil methyl ester (biodiesel) and its blends with diesel. Research is going on in right direction and likely to get surprising results.

Key words : Biodiesel, vegetable oil, methyl ester, diesel engine performance, emissions.

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 23-37

Effect of organochlorine pesticide Methoxychlor (1,1,1-trichloro-2,2-bis (4-methoxypheny1) ethane) on reproductive function of male albino rat

Suresh C. Joshi *, Rekha Goyal and Sunita Jain
Reproductive Toxicology Unit
Department of Zoology, University of Rajasthan
Jaipur – 302004 (India)

Abstract : The pesticide are one of the most potentially harmful chemicals liberated in the environment in an unplanned manner. Methoxychlor is widely used as a pesticide in many countries and has been shown to produce some adverse health effects. The present study was aimed to find the toxic effect of methoxychlor on reproductive system of male rats at the dose levels of 20, 25 and 30 mg /kg b.wt./day for 30 days. A significant reduction in the weight of testes and sex accessory organs was observed at all dose levels. The sperm motility in cauda epididymides and sperm density in cauda epididymides and testes were significantly decreased. The level of testosterone decreases in a dose depending manner. Biochemical results showed that acid phosphatase, alanine, aspartate amino transferases activity increased significantly, whereas alkaline phosphatase activity decreased. A significant reduction in sialic acid content of testes and sex accessory organs and testicular glycogen has been observed. Whereas, total protein content in testes and sex accessory organs and testicular cholesterol increased significantly. Results of present study clearly indicates the possibility of methoxychlor induced toxicity in male reproductive system of rats.

Key words : Organochlorine pesticides, methoxychlor, sperm dynamics, testosterone, biochemistry, male rat.
 

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 39-51

Isoproterenol hydrochloride Induced Protein Anabolic Effects in Denervated Chick Gastrocnemius Muscle

Surender S. Katoch and Chandrika Sharma
Department of Biosciences,
Himachal Pradesh University,
Summer Hill, Shimla – 171 005, INDIA

Abstract:  The study examined the effects of a maximum of 120 days treatment of male chicks (Gallus domesticus) with beta adrenoceptor agonist, isoproterenol hydrochloride in gastrocnemius muscle during early postnatal development. Four groups of chicks were maintained: Normal innervated control chicks (NI), Isoproterenol treated normal innervated chicks (NI + Iso; 1.5 mg/Kg body weight), Denervated chicks (Dn) and Isoproterenol treated denervated chicks (Dn + Iso). Post-treatment live body weights of normal innervated and denervated chicks were higher than control and denervated (-clenbuterol) chicks respectively. Measurement of dry muscle mass and protein content revealed a drug induced hypertrophy of normal innervated muscle and a reversal of atrophy in denervated muscle. Polyacrylamide gel electrophoresis (SDS-PAGE) of gastrocnemius myofibrillar proteins indicated a drug induced alteration in myosin and actin ratio. The data suggests that beta agonist exert its protein anabolic influences by reorganizing or remodeling myofibrillar proteins leading ultimately to a change in phenotypic expression of tissue.

Key Words : Chick, gastrocnemius muscle, isoproterenol hydrochloride, β- agonist, dry muscle mass, hypertrophy, myosin, actin.


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 53-58

Vitamin C against Concomitant Exposure to Heavy Metal and Radiation : A Study on Variations in Hepatic Cellular Counts

Sunita Gajawat, Garima Sancheti and P.K. Goyal
Radiation & Cancer Biology Laboratory,
Department of Zoology
University of Rajasthan, Jaipur-302 004, India

Abstract: The objective of this study was to screen for prophylactic use of ascorbic acid against radiation and heavy metal intoxication. For this purpose, male Swiss albino mice were intoxicated with lead acetate (20 mg/kg b. wt.) intraperitoneally 1 hr. before exposure to 4.80 Gy gamma radiation in the presence (experimental) or absence (control) of vitamin C (400 mg/kg b. wt.). Mice were sacrificed at various autopsy intervals (6 hrs. to 20 days) to examine quantitative changes in liver. It was observed that vitamin C administration, prior to combined treatment of lead acetate and radiation reduced the depletion in normal hepatocytes and the elevation in binucleate as well as abnormal hepatocytes in comparison to their respective controls, and moreover, it initiated a faster recovery to reinstate the normal cellular number by increasing glutathione level.

Key words : Metal toxicity, Gamma radiation, Chemical protection, Vitamin C, Liver, Swiss albino mice

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 59-62

A Study of Betel Leaf Microflora

Vivek Mishra 1 and Archana Mishra 2

1. Saifta P.G. College of Science and Education, Bhopal

2. Govt. MLB Girls College, Bhopal.

Abstract : The Betal vine (Piper betel) is a perennial dioecius creeper belonging to the family Piperaceae. The leaves of the plant have been traditionally used for chewing. Over thirty five varieties of betal leaves are cultivated in out country. They are grown in special Pan orchids known as ‘Barejas’ and have a complex system of cultivation, cropping and development. The study focuses on a comprehensive microbiological investigations of pathogen causing leaf diseases. It deals with pathogen isolation, pathogen classification, pathogen biochemical analysis and properties of pathogen culture. The study also compares the disease incidents on different betel leaf varieties and the losses caused to farmers and consumers. The present high rate of oral cancers in Bhopal underline the importance of the study.

Key Words : Piper betel, disease incidents, oral cancers, Microflora

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 63-68

Plant Regeneration from Immature Embryos of Indian Genotypes of Wheat

Anju Rao
Department of Botany
Panjab University
Chandigarh-160 014 (Panjab) India

Abstract : Immature embryos of 9 genotypes of T. aestivum L. and 6 of T. durum Desf. were cultured on MS, Murashige and Skoog; medium supplemented with 2.5 mg/l 2,4-D, 2,4-dichlorophenoxyacetic acid. After 4-5 weeks of culture initiation, embryogenic as well as non embryogenic callus was obtained. EC, Embryogenic callus; was pale, nodular and semi compact. EC, Embryogenic callus; was transferred to MS, Murashige and Skoog medium; medium supplemented with 2,4-D, 2,4-dichlorophenoxyacetic acid; (2.5 mg/l) +BAP, 6-benzylaminopurine; (1.0 mg/l) for plant regeneration. Regenerated plantlets were transferred to half-strength, hormone-free MS, Murashige and Skoog; medium for formation of well developed root system and then transferred to soil.

Key words : Immature embryo, Embryogenic callus, Plant regeneration, Indian genotypes.
 

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 69-76

Protective effect of Garlic on glutamate oxaloacetate transaminase and Glutamate pyruvate transaminase Enzymes of mice, Mus musculus intoxicated with Sevin

Mahira Parveen, Vinoy Kumar Shrivastava,
Mohammad Sanjay Qureshi & Santosh Kumar
Department of Biosciences
Barkatullah University, Bhopal, India

Abstract : In the present study, inhibition of liver enzymes of mice due to chronic exposure of sevin and their recovery by garlic treatment have been investigated. The sevin a highly potent pesticide causes serious hazardous effects on target enzymes phosphatases and oxaloacetates. Hepatotoxicity is the complex type of effect associated with sevin poisoning characterized by changes in metabolic and enzymatic activities. To understand the protective behavior of garlic pills in case of hepatotoxicity induced by a carbamate pesticide sevin, the work has been designed. Male mice, Mus musculus were divided into three groups. Group-1 included control animals. Group- 2 animals were treated with sevin for 15 days. Sevin dose of 2.5 mg /kg b.w. was injected intraperitoneally to animals every alternate days for the period of 15 days. Group -3 animals were treated with sevin for 15 days and cured with garlic for 15 days . Sevin was injected for 15 days followed by oral administration of garlic pills for 15 days. Sevin caused a decline in activities of Glutamate oxaloacetate transaminase and Glutamate pyruvate transaminase enzymes in liver of mice due to 15 days toxicity in group-2 as compared to control. Garlic treated mice recovered significantly as indicated by the beneficial rise in hepatic enzymes. This curing effect of garlic was found to be of clinical importance for the accidental sevin toxicity in mammals.

Key words : sevin, garlic, mice, liver


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 77-81

Guar Gum Grafting and It's Application to Textile

Ratna Sharma
Textile and Clothing
Sarojini Naidu Govt. Girls’ College
Bhopal (M.P.)

Abstract : It is a common belief that “natural” materials are more environmentally friendly than “artificial” or man made materials. Guar gum is naturally occurring non-ionic polysaccharide which is derived from the Guar seed “Gyamposis tetragonolobus”. Guar is commonly called as “gawar phalli”. This leguminous plant has been grown for centuries in India Guar is being grown in Madhya Pradesh in plenty and is mainly being used as food material for both man and animals. It is also used as viscosity builder and water binder in various industries. Guar gum forms viscous colloidal dispersion when hydrated in cold water. These are not very stable due to biodegradation that is why guar gum is rarely used in its natural forms. Drawback of guar gum may be minimized by grafting on to water soluble polyacrylamide using new redox initiating system. We have developed a new redox initiating system to examine the grafted solubility and viscosity of graft co-polymer. To add the knowledge of polymer science, either we can develop a new polymer from low-molecular weight compound by a known method or by modifying the existing natural and synthetic polymers either by derivation of their functional groups or grafting of different polymers or by blending\alloying with different materials. Existing natural and synthetic polymers are also modified by graft co-polymerization of various polymers onto their backbone.

Eco-Print System is water based printing system destined to meet standard with soft handle, bright colour and excellent fastness. The grafted guar gum was grinded to make powder and mixed with hot water. This product was tested with the dye “Reactive Brilliant Red H8B” against the usual gum paste. The product was used in printing of cotton fabric. The print so obtained from usual guar gum paste and from grafted guar gum paste have been verified as regards to their colour fastness by different tests from Hukumchand Mills Indore (M.P.)

Key Words: Guar Gum Grafting, Textile, Guar seed, Gyamposis tetragonolobus, Eco-Print System


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 83-92

Histopathological Study on the Liver of Channa punctatus treated with Sublethal Doses of Chlorpyrifos

B. L. Sharma 1 and D. P. Jaroli 2

1. Department of Zoology, Shri Bhawani Niketan Boys College, Jaipur, India
2. Department of Zoology, University of Rajasthan, Jaipur, India-302004.

Abstract : Present study is an attempt on the histopathology of liver of Channa punctatus after exposure of sublethal 0.037 ppm, 0.075 ppm and 0.110 ppm concentrations of an organophosphorous insecticide, chlorpyrifos (Dursban). The compound has been found to produce several damage in liver of Channa punctatus leading to various lesions like, cytoplasmic vacuolization, pycnosis, hepatic necrosis, karyorhexis, cirrhosis and finally failure of metabolic mechanism having resulted in the death of the fish. The severity of the damage has been found concentration and time dependent.

Key Words : Chlorpyrifos, Channa punctatus, picnosis, karyorhexis


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 93-103

Haemotoxic effects of Chocolate Brown, a commonly used blend of permitted food colour on Swiss Albino Mice

Anjali Sharma, R.P.Goyal*Geetanjali Chakravarty and Shipra Sharma
Department of Zoology
University of Rajasthan, Jaipur-302004

Abstract : The present study was conducted to evaluate the haemotoxic effects of Chocolate Brown, a commonly used food color on Swiss albino mice (Mus musculus) fed with a diet containing 2 gm/kg of body weight and 6 gm/kg of body weight of the dye for 21 days and 42 days as short term and long term, respectively. The blood parameter data showed a decrease in RBC count, TLC, haemoglobin content, haematocrit, MCH and MCHC values and slight increase in the values of MCV. Significant increase in the levels of alkaline phosphatase, triglycerides and proteins and decrease in the levels of cholesterol and glucose shows abnormal functioning of the liver.

Key words : Chocolate Brown, Mus musculus, haemotoxic


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 105-112

Adaptability of Membranes for Dairy Waste Management

A.S. Khojare, A.B. Kadu, P.G. Wasnik,M.R. Patil & Mrs. B. A. Khojare
College of Dairy Technology
[Maharashtra Animal & Fishery Sciences University, Nagpur]
Warud, (Pusad) – 445204 M.S. (India)

Abstract : Membranes produce a higher quality effluent than achieved using conventional filtration as a final polishing step in dairy Industry. Designed to simplify wastewater treatment, the membrane process replaces secondary clarifiers. Membranes are immersed directly in the bioreactor and operate at high levels of MLVSS (12,000-15,000 ppm) resulting in plant footprints up to 4 times smaller than conventional systems. This treatment process allows for long sludge retention times (up to 75 days), decreasing sludge production up to 70%. In addition the membranes are capable of being used with chemical treatment (phosphorus precipitation). The Membrane process offers the ideal solution for wastewater reuse applications, producing high quality effluent suitable for direct reuse in a single step process. The effluent is also ideally suited as an RO feed. Regardless of the feed water, membranes consistently produce an ideal RO feed, typically yielding a SDI < 3. This process provides significant advantages over conventional technologies where wastewater must be treated by a multi-step process prior to reuse. The development of electrically conducting membranes, which will control fouling and separation properties in the treatment of industrial effluent by adjusting surface charge. There is a real awareness that membrane technology will have a vital energy and environmental role to play in the dairy industry in the next ten years.

Key words: membrane, effluent treatment, dairy waste, zenon.


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 113-118

Quality Status of Potable Water of Tehsil-Sanganer, District-Jaipur, Rajasthan, India.

J.D. Sharma, M.K. Sharma, P. Jain and D. Sohu
Department of Zoology,
University of Rajasthan, Jaipur-302004, India.

Abstract : Drinking water quality of 17 villages of Sanganer Tehsil, Jaipur District, Rajasthan was analyzed to identify the nature of potability of water. The drinking water samples were collected in clean polyethylene one-liter cans and subjected for analysis in the laboratory. The parameters studied were pH, electrical conductivity (EC), fluoride (F), chloride (Cl), total dissolved solids (TDS), Ca and Mg hardness and total hardness (TH). The results revealed that the values of pH were found to be ranging between 6.39-8.34, EC ranged from 0.48-0.73 mmhos/cm and chloride content differed from 202.46-648.60 mg/l and total hardness varied from 413-1032 mg/l. The most important parameter fluoride was found to be 2.17-10.14 ppm, which is more than the permissible limit in all the samples studied. TDS varied from 82-429 mg/l. The data suggests that the drinking water of seventeen villages of Sanganer Tehsil contain high fluoride concentration, which leads to dreaded disease called fluorosis; hence drinking water is not potable.


Key words: Water quality, drinking water, electrical conductivity, fluoride, total dissolved solids, fluorosis.


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 119-126

Enzyme Inhibition (AChE) in Muscles and Skin of Oreochromis mossambicus due to Pesticidal Pollution of Herbicide “Pursuit”

Farhina Pasha 1 and Romsha Singh 2
1. Department of Biotechnology, Unique College, Bhopal (M.P.)
2. Department of Zoology, Govt. M. L. B. Girls P. G. (Autonomous) College, Bhopal (M.P.)

Abstract : In the present investigation the effect of three sub lethal concentrations of Pursuit i.e., 63.7 ppm, 85 ppm and 127.5 ppm in Oreochromis mossambicus were studied. Pursuit inhibited acetylcholinesterase of muscle and skin of Oreochromis mossambicus by increasing the Km and Vmax, thereby acting as a mixed inhibitor. The assay of muscle and skin AChE is thus useful for monitoring pesticide toxicity of fish.

Key words : Acetylcholinesterase, Oreochromis mossambicus, pursuit and mixed inhibitor.


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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 127-134

Vanadium Toxicity

B.V. Venkataraman and S. Sudha
Department of Pharmacology
St. John’s Medical College
Bangalore - 560034

Abstract : Vanadium is distributed extensively in nature. It is a trace element and is present in almost all-living organisms including man. Essentiality of this element in cellular functions is yet to be established. Biological importance of vanadium was originally recognised by its ability to inhibit membrane sodium pump. Its capacity to affect the activities of various other intracellular enzyme systems and to modify physiological processes is now documented. Vanadium is used extensively in various heavy industries. The incidence of exposure to toxic levels of vanadium to industrial workers has been an increasing concern for toxicologists. Disposition of vanadium in specific tissues may be involved in the pathogenesis of certain neurological disorders and cardiovascular diseases. An attempt is made to broadly document what is known of various biological/toxicological actions of vanadium.

Key words : Vanadium, CVS, Myocardium, Vascular smooth muscle, Respiratory system.

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Rapid Publication

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 135-141

New Eco-friendly Synthetic Procedures for the Reduction of Carbonyl Compounds

Sheesh Ram Yadav, Anil Kumar Nainawat, Shilpi Kaushik,
Alka Sharma and I. K. Sharma
*
Department of Chemistry
University of Rajasthan, Jaipur-302 004 (India)

Abstract : The bioreduction of some carbonyl compounds viz., Acetophenone, Benzalacetophenone, o-Aminoacetophenone, Benzil, Cinnamaldehyde, Crotonaldehyde and Salicylaldehyde was carried out with free Baker’s yeast (Saccharomyces cerevisiae) as well as Immobilized Baker’s yeast. The results of the bioreduction of these compounds have been compared. Merits of bioreduction have also been discussed.

Key words : Baker’s Yeast (BY), Immobilized Baker’s Yeast (ImBY), Bioreduction, Carbonyl Compounds.

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Commentary :-

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Asian J. Exp. Sci., Vol. 19, No. 2, 2005, 142

NECESSITY OF RADIATION EDUCATION BASED ON
NEW INFORMATION-

Tatsuo Matsuura
Radiation Education Forum, Masumoto-Bldg. Tokyo

At present, radiation and radionuclides are not only indispensable in medical diagnoses and treatments, but also are widely used in fundamental research and practical applications in various fields of science and technology, thus contributing much to us human beings for elevating the quality of life. Nuclear power production is also playing an important role in saving the nonrenewable natural energy resources, with the merit of minimizing the global warming. However, a majority of people, including many intellectuals, have an excessive concern for the risk of radiation and radioactivity even for very minute quantities. This fact is without doubt the consequence of following three reasons: (1) the first use of nuclear energy as the disastrous weapon in 1945 has resulted a profound socio-psychological after-effect, (2) the major accidents of nuclear power plants which occurred in the 1980‘s have been repeatedly reported in mass media with undue sensationalism, and (3) the proposition, that every ray of ionizing radiation may destroy the human cell and is harmful for human body such as by bringing carcinogenic or hereditary effects, has become a suspicion-free common sense for general public. This proposition has its basis on the recommendation by ICRP, which adopts the linear non-threshold (LNT) model for even very low dose of ionizing radiation(1). The majority of people cannot understand that this is only a hypothesis from the standpoint of radiation protection, but they believe this is a scientific truth. We believe there are ample evidences that the LNT model is no longer scientifically correct for interpreting the radiation effect in low dose, and this is the main topic of the present paper. At any rate, it is true that the majority of people show the syndrome of “radiophobia”.

If things will be left as they are, not only the proper use of radiation and the related nuclear disciplines in medicine and in many scientific and technological areas will be obstructed by the shortage of working personnel, but also the mankind may soon find difficulty in its existence by already threatening global warming and the very possible exhaustion of fossil fuel. The radiophobia is also producing various social demerits, such as the vast loss of money for the purpose of excessive protection for very low-level radiation, undue economical and socio-psychological loss due to ignorant rumor, and serious mental stress for radiation-exposed people. Thus the endeavor of radiation education of the public, i.e., by making the right knowledge about radiation and the related matters popular is keenly needed in global scale. As an approach to this aim, we established the “Radiation Education Forum”, a voluntary, non-profitable organization (NPO), composed of scientists and teachers, including distinguished researchers and expert radiologists, a few conscientious journalists and enthusiastic citizens in Japan, in 1994. We have continued various kinds of activities, focussing our energy mainly for improvement of radiation education in schools, because the social education has its basis on the school education.



 

   
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