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CONTENTS YEAR 2002
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Asian J. Exp. Sci., Vol. 16, No. 1&2,
2002, 1-8
Chronology of Meiosis and Spermiogenesis of
Mabuyia carinata
P. K. Mallick 1, T. Banerjee 2, S. C. Maity 2 and S. K. Ghosal 2
1. Department of Zoology, Dum Dum Motijheel
College, 1, Motijheel Avenue,
Kolkata – 700 074, West Bengal, India,
2. Molecular Genetics Lab., Department of
Zoology Burdwan University-713 104,
West Bengal, India.
Abstract: 3H-thymidine autoradiography of Mabuyia carinata spermatocytes
monitored sequentially revealed that the zygotene, diplotene, diakinesis
and metaphase stages is remarkably shorter (being individually hardly a day)
in contrast to prolonged pachytene (1.92 day), leptotene (1.40 day) and
spermiogenesis (9.00 day). Radioactive spermatozpa appear for the first time
at 14.52 d.p.i. Phylogenetically the total duration increases from fish to
mammals as regards meiosis and spermiogenesis.
Key Wards : Gamatogenesis, Reproductive physiology, Chronobiology,
Spermatogenesis.
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Asian J. Exp. Sci., Vol. 16, No. 1&2,
2002, 9-19
Spilanthes acmella Murr. : Study on Its
Extract Spilanthol as Larvicidal Compound
D.K. Saraf 1 and V.K. Dixit 2
1. Reader, Department of Zoology,
Dr. H. S. Gour University, Sagar, M. P.
2. Department of Pharmaceutical Sciences,
Dr. H. S. Gour University, Sagar, M. P.
Abstract: Spilanthol, a major constituent of ethanolic extract of flower heads of
Spilanthes acmella Murr. is having potent ovicidal, larvicidal and pupicidal
activity. Maximum 7.5 ppm concentration causes 100% motility of eggs, larvae
and pupae of Anopheles, Culex and Aedes mosquito. Spilanthol is more
effective even at low doses against eggs and pupae. In pupae, it seems to
work on nervous system which was evident by abnormal movement like jerks,
spinning and uncoordinated muscular activity suggesting thereby that it
disturbs nerve conduction.
Key Words : Spilanthol, Spilanthes acmella, Larvicidal, Ovicidal,
Culex quifaciatus, Say, Anopheles culicifacies, Giles and Aedes aegypti,
Linn.
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Asian J. Exp. Sci., Vol. 16, No. 1&2,
2002, 21-28
Screening of Leaf Extracts of Some Plants for
Their Nematicidal and Fungicidal Properties Against Meloidogyne incognita
and Fusarium oxysporum
Nidhi Sharma and P.C. Trivedi
Department of Botany
University of Rajasthan, Jaipur -302 004
Abstract: Fresh leaf extracts of Datura stramonium,
Calotropis procera, Verbesena enceloides, Parthenium hysterophorus, Morus
alba, Phyllanthus amarus, Eichhornea crassipes, Ricinus communis, Jatropha
curcas, Azadirachta indica, Tinospora cordifolia, Clerodendron multiflorum,
Catharanthus roseus and Adhatoda vesica were tested against
root-knot nematode, Meloidogyne incognita and wilt
fungus, Fusarium oxysporum f.sp. cumini infesting cumin. In the
preliminary studies, almost all the plant species exhibited nematicidal and
antifungal property. Calotropis procera and Ricinus
communis gave best results against the nematode and Datura
stramonium and Calotropis procera showed maximum
antifungal activity against Fusarium oxysporum f.sp. cumini.
Key Words : Fusarium oxysporum f.sp. cumini, Meloidogyne
incognita, Trichoderma viride, T. harzianum, T. virens, T. hamatum.
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Asian J. Exp. Sci, Vol. 16, No. 1&2, 2001, 29-34
Evaluation of Leaf Extracts for their Ovicidal
Action Against Callosobruchus chinensis (L.)
S.C. Dwivedi and Sonia Venugopalan
Ecotoxicology Laboratory
Department of Zoology
University of Rajasthan,
Jaipur - 302004. India
Acetone extracts of seven plant species
Tamarindus indica Linn., Ficus indica Linn., Ficus religiosa
Linn., Tabernaemontana livaricate Linn., Murraya koenigii Linn., Chenopodium
album Linn., Syzygium cuminii Linn. were evaluated for their
ovicidal action. Murraya Tabernaemontana and Chenopodium showed 70%, 75% and
66.6% ovicidal action at 100% dose level whereas at the same dose level T.
Indica, F. indica, F. religiosa and S. cuminii showed 48.3%, 41.6%, 13.3%,
53.3% ovicidal action respectively.
Key Words :- Leaf extracts, ovicidal action, Callosobruchus
chinensis (L.).
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Asian J. Exp. Sci., Vol. 16, No. 1&2, 2002, 35-39
Biochemical Alterations in the Haemolymph of
Bacillus thuringiensis var. kurstaki (B.t.k.) Infected Larvae of Spodoptera
litura (Fab)
Rashmi Tripathi and N.P. Singh
Department of Zoology
University of Rajasthan,
Jaipur - 302 004, Rajasthan
The effect of Bacillus thuringiensis var.
kurstaki (B.t.k.) on pathophysiology of Spodoptera litura
(fab.) larvae was studied at 24, 28 and 72 hour post infection (hpi).
The infection resulted in significant alterations in various biochemical
parameters of haemolymph, protein, carbohydrate, uric acid and lipid
contents were higher in treated larvae as compared to untreated control. The
increase was maximum for III, IV and V instar larvae at 72 hpi in case of
protein, carbohydrate, lipid and uric acid content.
Key words : Bacillus thuringiensis var. kurstaki (B.t.k.)
Spodoptera litura, Biopesticide, Haemolymph.
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Asian J. Exp. Sci., Vol. 16, No. 1&2, 2002, 41-49
Spectrophotometeric and Titrimetric Analysis
of Lipid Peroxidation Products in Living Tissues
Pallavi Mishra 1 and K. Manda
2
1. Department of Chemistry, JNV University,
Jodhpur-Rajasthan
2. Deparrtmentr of Zoology, University of Rajasthan, Jaipur
A number of methods are available to measure
lipid peroxidation, but no single assay is an accurate measure of the whole
oxidation process. Present study therefore an attempt to analyze the
different lipid peroxidation products viz. Malondialdehyde, Hydroperoxide
and Diene conjugates by simple spectrophotometric and titrimetric
techniques. To justify the accuracy and reproducibility of results, I
compared the values of lipid peroxidation products of old animal tissues
with the young animals.
Key words: Lipid peroxidation, Free radicals, Oxidative stress,
Malondialdehyde, Hydroperoxide and Diene conjugates.
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Asian J. Exp. Sci, Vol. 16, No. 1&2,
2002, 51-63
A Comparative Study of Effect of Alcoholic
Extracts of Sapindus emarginatus, Terminalia belerica, Cuminum cyminum and
Allium cepa on Reproductive Organs of Male Albino Rats
V. Venkatesh, J. D Sharma 1 and Raka Kamal 2
1. Laboratory of Reproductive Physiology,
Department of Zoology,
University of Rajasthan, Jaipur - 302004, India
2. Department of Botany, University of Rajasthan,
Jaipur 302004, India
Fruit extracts of S. emarginatus, T. belerica and seed
extracts of Cuminum cyminum and Allium cepa (50
mg/day/rat) were fed orally to male albino rats for 60 days. The body
weights were not affected but the weights of reproductive organs were
decreased following the treatments. The sperm motility of cauda epididymis
and sperm count of cauda epididymis and testis declined significantly
leading to negative fertility test. Androgen dependent parameters (protein,
sialic acid, fructose and ascorbic acid) were lowered, revealing reduction
in the circulating androgen but on the contrary there was an increase in the
testicular cholesterol level. Haemoglobin and Haematocrit values remained
unaltered, indicating normal blood physiology of treated groups of animals.
Serum biochemistry indicated an increase in the lipid parameters. The
present study therefore, suggests that these extracts exert both
antifertility and antiandrogenic activities. The overall data suggested S.
emarginatus is superior to other three plant extracts in exerting the
antifertility effects.
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