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CONTENTS YEAR 2001
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Asian J. Exp. Sci, Vol. 15, No. 1&2,
2001, 1-8
Effect of Different Inoculum Levels
of Nematode, Heterodera avenae on Photosynthetic Efficiency of Barley (Hordeum
vulgare L.)
Sampat Nehra, Indu Rani Sharma and P.C. Trivedi
Department of Botany
University of Rajasthan, Jaipur - 302 004, India
Under present investigation effect of different
inoculum levels of H. avenae on the rate of photosynthesis and water
absorption capacity of barley variety RD 103 was evaluated. Photosynthetic
efficiency of Hordeum vulgare in terms of Absolute Growth Rate
(AGR), Relative Growth Rate (RGR), Net Assimilation Rate (NAR) and
chlorophyll content was recorded in healthy and diseased plants of
susceptible barley variety (RD 103) during growth periods of 30-60, 60-90
days. Total chlorophyll content and net photosynthetic rate decreased with
increasing inoculum levels in diseased plants over their healthy control.
Reduction in AGR was evident during growth periods of 30-60 and 60-90 at
10,000 inoculum level but in 90-120 days old plants even at the inoculum
level of 100 juveniles per kg soil. Maximum decrease in RGR was noted at
10,000 inoculum level at all the growth periods over healthy checks.
Similarly in 30-60 days old plants NAR was minimum at 10,000 inoculum level,
whereas in 60-90 and 90-120 days old plants it was minimum at 1000 and 100
inoculum levels respectively.
Key Words : H. avenae, Hordeum
vulgare, Absolute Growth Rate (AGR), Relative Growth Rate (RGR), Net
Assimilation Rate (NAR), Chlorophyll, Photosynthesis etc.
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Asian J. Exp. Sci, Vol. 15, No. 1&2,
2001, 9-16
Molecular Mechanisms Underlying the Inhibition of Cell Proliferation by
Cleistanthins
S. Rajkumar1, A.L. Bhatia3, G. Shanmugam2
1 Iladevi Cataract and IOL Research Centre, Gurukul, Ahmedabad, India.
2 Cancer Biology Division, School of Biological Sciences, Madurai
Kamaraj University,
Madurai, India.
3 Department of Zoology, University of Rajasthan, Jaipur, India.
Cleistanthus collinus, a toxic
plant, contains three glycosidic compounds, Cleistanthin A, B and C.
Cultured K562 cells were treated with 1mg/ml and 10mg/ml of each
Cleistanthins (A and B). At higher concentration a significant dose
dependent decrease has been noted in the incorporation of thymidine and
uridine into DNA and RNA, respectively. However, no significant difference
in leucine incorporation into proteins was discerned. Hence present study
shows Cleistanthin A and B inhibition nucleotides (DNA, RNA) with no
difference in aminoacid transportation into macromolecules (Protein) into
cell cytoplasm of K562 cells. However it dos not affect transport of
nucleotides and aminoacids into the cytoplasm. Thus, these results showed
that the cell growth/ proliferation is checked by these compounds at the
replication and transcriptional level, but not at the translational level.
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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 17-26
Seasonal Changes in Free Amino Acid
Contents in Lateral Muscles and Anterior Kidney of the Teleost Fish, Nandus
Nandus Cuv.
D.K.Saraf
Department of Zoology,
Dr. Sri Hari Singh Gour University,
Sagar, M.P.
The free amino acids in the lateral muscles and
anterior kidney are estimated both, qualitatively and quantitatively. The
free amino acid pool of the lateral muscles exhibits almost definite
constancy. The pool is observed to be disturbed due to occasional appearance
of some amino acids. Roughly 5% amino acids in this tissue are extra
additions during the course of the year. The maximum alterations are seen in
the spawning season in the tissue studied. Tyrosine, tryptophan and
ornithine appear only in the breeding season. The highest concentration of
the free amino acids is anticipated in the spawning period. A continuous
gradual increase in the quantity of the free amino acids is observed up to
spawning period. In anterior kidney, besides other amino acids, arginine and
ornithine are also detected. The results point to the fact that the amino
acid pool in the fish tissues may be almost invariable yet quantity wise it
is alternating according to breeding season. The amino acids in the
intercellular fluid help to maintain the milieu and are taken up by the
cells when required for some metabolic activity.
Key words : Free amino acids,
Seasonal variations, Lateral muscles, Anterior kidney, Fish Nandus nandus
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Asian J. Exp. Sci, Vol. 15, No. 1&2,
2001, 27-37
Effects of Some Indigenous Indian
Medicinal Preparations on Cognitive Functions
Gyan Vikash Mishra* and S. B. Vohora#
* P G Deptt. of Zoology, Govt. College, Dungarpur (Rajasthan)
# Deptt. of Medical Elementology and Toxicology Hamdard University, New
Delhi.
In Indian indigenous systems of medicines
namely Ayurveda and Unani-Tibb some mineral preparations are
used and called as Bhasmas and Kushta
respectively. Preparations of these drugs involve calcination of certain
metals and incorporation of some herbal juices into it through some
exhaustive procedures. In the present study four such drugs have been
evaluated for their toxic manifestations on cognitive functions. Two drugs
are the calcined forms of mercury and two are of lead taken one each from
Ayurvedic and Unani systems. Ayurvedic preparations were Kajjali
Bhasma (Hg) and Nag Bhasma (Pb), and Unani drugs were
Kushta-Sangraf (Hg) and Kushta-Surb (Pb). For testing the cognitive activity
two behavioural models of active and passive learning with retention were
used in the study. To substantiate these findings neurochemical estimation
of acetylcholinesterase and serotonin in discrete brain areas were also
carried out. All the four drugs caused the impairment of cognitive functions
along with corresponding effects on neurochemical parameters on high dose in
ten days study. Result in this study calls for some extra precautions while
prescribing such extensively used preparations.
Key Words: Kushta Sangraf (KS-I), Kushta Surb (KS-II), Kajjali
Bhasma (KB), Nag Bhasma (NB), Learning Score, and Retention
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Asian J. Exp. Sci, Vol. 15, No. 1&2,
2001, 39-47
Radioprotective Role of Diltiazem on
Phosphatase Activity in Brain of Swiss Albino Mice After Exposure to Gamma
Radiation
Rekha Yadav and P.K. Goyal
Radiation & Cancer Biology Laboratory,
Department of Zoology
University of Rajasthan, Jaipur –302 004
Diltiazem, a benzathiazapine (calcium channel blocker) used widely in
cardiovascular therapy, protected acid and alkaline phosphatase activities
in mouse brain against ionizing radiation. A dose of 100 mg/kg b.wt. of
diltiazem (DTZ) was injected intraperitoneally in mice half an hour before
2.5 Gy gamma irradiation. The values of acid phosphatase were found to be
significantly higher than the normal throughout the experiment in without
DTZ treated irradiated group (control). Diltiazem pretreated irradiated mice
(experimental) showed significant decrease towards normal in acid
phosphatase activity as compared to control. Conversely, a pronounced
decline in alkaline phosphatase activity was seen in both the groups, but in
diltiazem pretreated irradiated group, values were observed as significantly
higher than control at early intervals. Thus, the present results indicate
radiprotective role of diltiazem in phosphatases activities of brain in
mice.
Keywords : Radioprotection, Diltiazem,
Acid-alkaline Phosphatase, Gamma irradiation
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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 49-62
Thermodynamics Studies of Zinc
Ethylenediamine Complexes in Aqueous-Non-Aqueous Media by Polarography
R.S. Pandey and Sushma Dugar
Department of Chemistry
University of Rajasthan, Jaipur-302004, Rajasthan
Behavior of the complex Zinc (II)
Ethylenediamine in Dimethylformamide-Water and Isopropanol-Water mixture
studied by direct current polarography. The reduction of complex in both the
mixtures was found to be diffusion controlled as revealed by constant value
of id/h˝eff, and quassi-reversible also as the slope value varies from 51mV
to 54mV. The Er˝ values were determined using Gellings graphical methods and
the shifting of Er˝ towards more negative side with successive addition of
the ligand to the metal and decreased diffusion current in all the mixture
of the solvent [20%, 40% and 60% (by volume) DMF- water and isopropanol-water
mixtures] confirm complexation between metal and ligand in them.
Key words : Direct current polarography, Complexation, Znic (II)
Ethylenediamine, Aqueous-non-aqueous media.
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