AJES
Asian Journal of Experimental Sciences
 
 
AJES

 

 


CONTENTS YEAR 2001

Click on the linked title to view the paper

 

Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 1-8

Effect of Different Inoculum Levels of Nematode, Heterodera avenae on Photosynthetic Efficiency of Barley (Hordeum vulgare L.)

Sampat Nehra, Indu Rani Sharma and P.C. Trivedi
Department of Botany
University of Rajasthan, Jaipur - 302 004, India

Under present investigation effect of different inoculum levels of H. avenae on the rate of photosynthesis and water absorption capacity of barley variety RD 103 was evaluated. Photosynthetic efficiency of Hordeum vulgare in terms of Absolute Growth Rate (AGR), Relative Growth Rate (RGR), Net Assimilation Rate (NAR) and chlorophyll content was recorded in healthy and diseased plants of susceptible barley variety (RD 103) during growth periods of 30-60, 60-90 days. Total chlorophyll content and net photosynthetic rate decreased with increasing inoculum levels in diseased plants over their healthy control. Reduction in AGR was evident during growth periods of 30-60 and 60-90 at 10,000 inoculum level but in 90-120 days old plants even at the inoculum level of 100 juveniles per kg soil. Maximum decrease in RGR was noted at 10,000 inoculum level at all the growth periods over healthy checks. Similarly in 30-60 days old plants NAR was minimum at 10,000 inoculum level, whereas in 60-90 and 90-120 days old plants it was minimum at 1000 and 100 inoculum levels respectively.

Key Words : H. avenae, Hordeum vulgare, Absolute Growth Rate (AGR), Relative Growth Rate (RGR), Net Assimilation Rate (NAR), Chlorophyll, Photosynthesis etc.

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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 9-16

Molecular Mechanisms Underlying the Inhibition of Cell Proliferation by Cleistanthins

S. Rajkumar1, A.L. Bhatia3, G. Shanmugam2
1  Iladevi Cataract and IOL Research Centre, Gurukul, Ahmedabad, India.
2  Cancer Biology Division, School of Biological Sciences, Madurai Kamaraj University,
    Madurai, India.
3  Department of Zoology, University of Rajasthan, Jaipur, India.

Cleistanthus collinus, a toxic plant, contains three glycosidic compounds, Cleistanthin A, B and C. Cultured K562 cells were treated with 1mg/ml and 10mg/ml of each Cleistanthins (A and B). At higher concentration a significant dose dependent decrease has been noted in the incorporation of thymidine and uridine into DNA and RNA, respectively. However, no significant difference in leucine incorporation into proteins was discerned. Hence present study shows Cleistanthin A and B inhibition nucleotides (DNA, RNA) with no difference in aminoacid transportation into macromolecules (Protein) into cell cytoplasm of K562 cells. However it dos not affect transport of nucleotides and aminoacids into the cytoplasm. Thus, these results showed that the cell growth/ proliferation is checked by these compounds at the replication and transcriptional level, but not at the translational level.


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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 17-26

Seasonal Changes in Free Amino Acid Contents in Lateral Muscles and Anterior Kidney of the Teleost Fish, Nandus Nandus Cuv.

D.K.Saraf

Department of Zoology,
Dr. Sri Hari Singh Gour University,
Sagar, M.P.

The free amino acids in the lateral muscles and anterior kidney are estimated both, qualitatively and quantitatively. The free amino acid pool of the lateral muscles exhibits almost definite constancy. The pool is observed to be disturbed due to occasional appearance of some amino acids. Roughly 5% amino acids in this tissue are extra additions during the course of the year. The maximum alterations are seen in the spawning season in the tissue studied. Tyrosine, tryptophan and ornithine appear only in the breeding season. The highest concentration of the free amino acids is anticipated in the spawning period. A continuous gradual increase in the quantity of the free amino acids is observed up to spawning period. In anterior kidney, besides other amino acids, arginine and ornithine are also detected. The results point to the fact that the amino acid pool in the fish tissues may be almost invariable yet quantity wise it is alternating according to breeding season. The amino acids in the intercellular fluid help to maintain the milieu and are taken up by the cells when required for some metabolic activity.

Key words : Free amino acids, Seasonal variations, Lateral muscles, Anterior kidney, Fish Nandus nandus

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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 27-37

Effects of Some Indigenous Indian Medicinal Preparations on Cognitive Functions

Gyan Vikash Mishra* and S. B. Vohora#
* P G Deptt. of Zoology, Govt. College, Dungarpur (Rajasthan)
# Deptt. of Medical Elementology and Toxicology Hamdard University, New Delhi.

In Indian indigenous systems of medicines namely Ayurveda and Unani-Tibb some mineral preparations are used and called as Bhasmas and Kushta respectively. Preparations of these drugs involve calcination of certain metals and incorporation of some herbal juices into it through some exhaustive procedures. In the present study four such drugs have been evaluated for their toxic manifestations on cognitive functions. Two drugs are the calcined forms of mercury and two are of lead taken one each from Ayurvedic and Unani systems. Ayurvedic preparations were Kajjali Bhasma (Hg) and Nag Bhasma (Pb), and Unani drugs were Kushta-Sangraf (Hg) and Kushta-Surb (Pb). For testing the cognitive activity two behavioural models of active and passive learning with retention were used in the study. To substantiate these findings neurochemical estimation of acetylcholinesterase and serotonin in discrete brain areas were also carried out. All the four drugs caused the impairment of cognitive functions along with corresponding effects on neurochemical parameters on high dose in ten days study. Result in this study calls for some extra precautions while prescribing such extensively used preparations.

Key Words: Kushta Sangraf (KS-I), Kushta Surb (KS-II), Kajjali Bhasma (KB), Nag Bhasma (NB), Learning Score, and Retention

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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 39-47

Radioprotective Role of Diltiazem on Phosphatase Activity in Brain of Swiss Albino Mice After Exposure to Gamma Radiation

Rekha Yadav and P.K. Goyal
Radiation & Cancer Biology Laboratory,
Department of Zoology
University of Rajasthan, Jaipur –302 004

Diltiazem, a benzathiazapine (calcium channel blocker) used widely in cardiovascular therapy, protected acid and alkaline phosphatase activities in mouse brain against ionizing radiation. A dose of 100 mg/kg b.wt. of diltiazem (DTZ) was injected intraperitoneally in mice half an hour before 2.5 Gy gamma irradiation. The values of acid phosphatase were found to be significantly higher than the normal throughout the experiment in without DTZ treated irradiated group (control). Diltiazem pretreated irradiated mice (experimental) showed significant decrease towards normal in acid phosphatase activity as compared to control. Conversely, a pronounced decline in alkaline phosphatase activity was seen in both the groups, but in diltiazem pretreated irradiated group, values were observed as significantly higher than control at early intervals. Thus, the present results indicate radiprotective role of diltiazem in phosphatases activities of brain in mice.

Keywords : Radioprotection, Diltiazem, Acid-alkaline Phosphatase, Gamma irradiation

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Asian J. Exp. Sci, Vol. 15, No. 1&2, 2001, 49-62

Thermodynamics Studies of Zinc Ethylenediamine Complexes in Aqueous-Non-Aqueous Media by Polarography

R.S. Pandey and Sushma Dugar
Department of Chemistry
University of Rajasthan, Jaipur-302004, Rajasthan

Behavior of the complex Zinc (II) Ethylenediamine in Dimethylformamide-Water and Isopropanol-Water mixture studied by direct current polarography. The reduction of complex in both the mixtures was found to be diffusion controlled as revealed by constant value of id/h˝eff, and quassi-reversible also as the slope value varies from 51mV to 54mV. The Er˝ values were determined using Gellings graphical methods and the shifting of Er˝ towards more negative side with successive addition of the ligand to the metal and decreased diffusion current in all the mixture of the solvent [20%, 40% and 60% (by volume) DMF- water and isopropanol-water mixtures] confirm complexation between metal and ligand in them.

Key words : Direct current polarography, Complexation, Znic (II) Ethylenediamine, Aqueous-non-aqueous media.

 

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