Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 1-4
 

Identification of Phenolic Component of Dettol and Phenyle in Visceral Material

M. Sodhiya, V. Dhingra, J. Pandey and A.K Guru
Forensic Science Laboratory
Sagar - 470003 (M.P.)

The present study deals with the use of thin layer chromatography (TLC) technique for the detection of o-, m-, p-cresols, phenol, Lysol and chloro-xylenol in some of the household disinfectants viz. Phenyle and Dettol preparation and biological materials viz. cresols, phenol, Lysol and chloroxylenol have been detected on silica gel coated TLC plates by spraying chromogenic reagent 4-aminoantipyrine. The method described here is sensitive and give colour test for detection of constituents of 'Dettol' and 'Phenyle' present in the vomit, stomach wash, cold/alcoholic drinks, visceral materials and in other edible materials.

Key words : TLC, Phenols, Phenyle, Dettol, Household Disinfectants.
 

Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 5-8

Identification of Glyphosate in Biological Materials by Thin-Layer Chromatography-A Thin Layer Chromatographic Study

M. Sodhiya, V. Dhingra, J.Pandey and A.K Guru 7-10
Forensic Science Laboratory
Sagar - 470003 (M.P.)

Glyphosate N-(Phosphonomethyl) Glycine is a herbicide used in field crops. Easy availability of this compound frequently encountered in forensic case work. The present study deals with the use of thin-layer chromatography for the detection of Glyphosate in biological materials. Glyphosate detected on silica gel coated TLC plates in different solvent systems, using spraying reagent potassium bismuth iodide and Ninhydrin solutions. This technique is easy and useful in the detection of Glyphosate in biological materials in forensic study.

Key words : Herbicide, Glyphosate, Glycel, N-(Phosphonomethyl) Glycine. Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 9-11
 

Acid and Alkaline Phosphatase Activity in the Midgut Tissue of Spodoptera litura (Fab.) (Lepidoptera : Noctuidae), Infected with Nuclear Polyhedrosis Virus

V. Kumari and N.P. Singh
Department of Zoology,
University of Rajasthan, Jaipur - 302004
Rajasthan (India)

Changes in the activity of acid and alkaline phosphatase in Spodoptera litura larvae infected with nuclear polyhedrosis virus (Sl NPV) have been investigated. The enzyme activity of acid and alkaline phosphatases in the midgut tissue of 24, 48 and 72 hours post infected (hpi) larvae was significantly higher than that of uninfected larvae. The increase was maximum for alkaline phosphatase at 72 hpi for 5 day and Acid phosphatase at 48 hpi for 8 day old larvae.

Key words : Acid phosphatase, Alkaline phosphatase, midgut, Spodoptera litura (Fab.), nuclear polyhedrosis virus.
 

Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 13-18

Field Evaluation of Anticoagulants Against Poison-shy Population of Rodents

A. Mathur and A. Saxena
Department of Zoology
Raj Rishi College, Alwar (Rajasthan)

Field evaluation of anticoagulants, Brodifacoum and Difethialone reveal that both the anticoagulants are highly effective to control the poison-shy population of rodents. After the intermittent treatment of zinc phosphide (single and double exposure) 93.3 % and 92.8% control success was recorded respectively with Brodifacoum (0.005% conc.) while Difethialone treatment gave 90.6% and 92.3 % control success after single and double exposure of 2 % zinc phosphide.

Key words : Anticoagulants, Brodifacoum, Difethialone, zinc phosphide.
 

Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 19-25

Laboratory efficacy of Anticoagulants against Poison-shy population of Mus musculus albino

A. Mathur and A. Saxena
Department of Zoology
Raj Rishi College, Alwar (Rajasthan)

The efficacy of anticoagulants, Brodifacoum and Difethialone on poison shy albino mice ( with a prior feeding of sub lethal dose of zinc phosphide ) reveal a markedly high suspectibility in no choice bait feeding with a low range of days (2-4 with 0.005% conc. Brodifacoum and 3-7 days with 0.000625 % conc. Difethialone). The acute median lethal dose, LD50 for poison shy albino mice also showed an increase oral toxicity after an prior ingestion of zinc phosphide.

Key words : Anticoagulants/Brodifacoum/Difethialone/Poison-shy population, Oral Toxicity.
 

Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 27-35

Significance of Serum Copper, Zinc and Copper/Zinc Ratio in Cancer Head and Neck and Cervix Uterus Patients Treated with Radiotherapy

A. Chougle and S. Hussain**
*Department of Radiotherapy
RNT Medical College, Udaipur - 313 001
**Meera Girls College, Udaipur

The Trace elements such as copper (Cu) and Zinc (Zn) are very essential for normal physiological activities of human being. Inadequate levels of these trace elements may cause disfunctioning or illness. We have estimated serum copper levels (SCL) and serum zinc levels (SZL) in 70 patients with head and neck malignancy and 57 patients with malignancy of cervix uterus. The estimations were done on atomic absorption spectrometer [902/903 GBC] in blood samples collected before start of radiotherapy, midway between radiotherapy (30 Gy tumor dose), at completion of therapy (60-70 Gy tumor dose) and during subsequent monthly follow-ups. Estimation were also done in control group of 20 healthy individuals and were 109.5 ± 18.02 µg/100ml, 84.05 ± 12.3 µg/100 ml and 1.27 ± 0.30 for SCL, SZL and Cu/Zn ratio respectively. In the present study we observed significantly higher levels of copper in both head and neck and cervix malignancies . In head and neck the copper levels before start of radiotherapy were 234.1 ± 28.1, 257.2 ± 24.8, 281.2 ± 25.6 (µg/100ml) for stage IIA, IIB and IIA respectively. Similarly the levels in cancer cervix were 263.8 ± 13.0, 305.5 ± 11.5, 312.2 ± 54.7 (µg/100ml)for stage II, IIIA and IIB respectively . The SZL were lower than the control group in both malignancies of head and neck and cervix. We further found that the SCL and Cu/Zn ratio to decrease as tumor regresses where as SZL levels were observed to increase. The Cu/Zn ratio can be used to predict the prognosis of the disease to radiotherapy in head and neck and cervix malignancies.

Key words: Copper, Zinc, Malignancy, Copper/Zinc, Tumor regression, Biochemical investigation
 

Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 37-46

Management of Malignancies by Radiotherapy and Role of Biochemical Parameters

A. Chougule, S. Hussain* and D.P. Singh**
Department of Radiotherapy
SMS Medical College and Hospital,
Jaipur-302 004

*Meera Girls College,
Udaipur-313 001

**RNT Medical College,
Udaipur-313 001

There is fast accumulating evidence that environmental factors play a very significant role in occurrence of malignancy. The environmental factors alter cellular growth leading to biochemical changes in blood serum. In the present communication we have made an attempt to evaluate the biochemical changes such as macrometals (magnesium and calcium), serum pseudocholine esterase (PCHE) and serum lactate de hydrogenase (LDH) levels as side adjuct in diagnosis and prognosis of 126 biopsy proved malignancies of head & neck and cervix of uterus.

In the present communication we observed that PCHE levels were within normal limits in early stage of malignancy whereas they were significantly lowered in advanced stage of malignancy. As radiotherapy progressed the level again tend towards normal limit. The LDH activity was much higher than the normal limits in all the cases of malignancy. The LDH activity became normal after completion of radiotherapy in all the patients with no tumor activity. Hyper calcemia and hyper magnesia was observed in advanced malignancy and level decreased as radiotherapy progressed. From present study we conclude that the biochemical tests in combination can be employed to know the extent of tumor activity and has got excellent prognostic significance.

Key words: Malignancy, Biochemical Markers, Hypercalcemia