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CONTENTS YEAR 1999
1. |
Asian J. Exp. Sci,
Vol. 13, No. 1&2, 1999, 1-4
Identification of Phenolic Component of Dettol and Phenyle in
Visceral Material
M. Sodhiya, V. Dhingra, J. Pandey and A.K Guru
Forensic Science Laboratory
Sagar - 470003 (M.P.)
The present study deals with the use of thin layer
chromatography (TLC) technique for the detection of o-, m-,
p-cresols, phenol, Lysol and chloro-xylenol in some of the
household disinfectants viz. Phenyle and Dettol preparation and
biological materials viz. cresols, phenol, Lysol and
chloroxylenol have been detected on silica gel coated TLC plates
by spraying chromogenic reagent 4-aminoantipyrine. The method
described here is sensitive and give colour test for detection
of constituents of 'Dettol' and 'Phenyle' present in the vomit,
stomach wash, cold/alcoholic drinks, visceral materials and in
other edible materials.
Key words :
TLC, Phenols, Phenyle, Dettol, Household Disinfectants.
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2. |
Asian J. Exp. Sci,
Vol. 13, No. 1&2, 1999, 5-8
Identification
of Glyphosate in Biological Materials by Thin-Layer
Chromatography-A Thin Layer Chromatographic Study
M. Sodhiya, V. Dhingra, J.Pandey and A.K Guru 7-10
Forensic Science Laboratory
Sagar - 470003 (M.P.)
Glyphosate N-(Phosphonomethyl)
Glycine is a herbicide used in field crops. Easy availability of
this compound frequently encountered in forensic case work. The
present study deals with the use of thin-layer chromatography
for the detection of Glyphosate in biological materials.
Glyphosate detected on silica gel coated TLC plates in different
solvent systems, using spraying reagent potassium bismuth iodide
and Ninhydrin solutions. This technique is easy and useful in
the detection of Glyphosate in biological materials in forensic
study.
Key words :
Herbicide, Glyphosate, Glycel, N-(Phosphonomethyl) Glycine.
Asian J. Exp. Sci, Vol. 13, No. 1&2, 1999, 9-11
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3. |
Acid and Alkaline Phosphatase
Activity in the Midgut Tissue of Spodoptera litura (Fab.)
(Lepidoptera : Noctuidae), Infected with Nuclear Polyhedrosis
Virus
V. Kumari and N.P. Singh
Department of Zoology,
University of Rajasthan, Jaipur - 302004
Rajasthan (India)
Changes in the
activity of acid and alkaline phosphatase in Spodoptera litura
larvae infected with nuclear polyhedrosis virus (Sl NPV) have
been investigated. The enzyme activity of acid and alkaline
phosphatases in the midgut tissue of 24, 48 and 72 hours post
infected (hpi) larvae was significantly higher than that of
uninfected larvae. The increase was maximum for alkaline
phosphatase at 72 hpi for 5 day and Acid phosphatase at 48 hpi
for 8 day old larvae.
Key words :
Acid phosphatase, Alkaline phosphatase, midgut, Spodoptera
litura (Fab.), nuclear polyhedrosis virus.
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4. |
Asian J. Exp. Sci,
Vol. 13, No. 1&2, 1999, 13-18
Field Evaluation of
Anticoagulants Against Poison-shy Population of Rodents
A. Mathur and A. Saxena
Department of Zoology
Raj Rishi College, Alwar (Rajasthan)
Field evaluation of
anticoagulants, Brodifacoum and Difethialone reveal that both
the anticoagulants are highly effective to control the
poison-shy population of rodents. After the intermittent
treatment of zinc phosphide (single and double exposure) 93.3 %
and 92.8% control success was recorded respectively with
Brodifacoum (0.005% conc.) while Difethialone treatment gave
90.6% and 92.3 % control success after single and double
exposure of 2 % zinc phosphide.
Key words :
Anticoagulants, Brodifacoum, Difethialone, zinc phosphide.
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5. |
Asian J. Exp. Sci,
Vol. 13, No. 1&2, 1999, 19-25
Laboratory
efficacy of Anticoagulants against Poison-shy population of Mus
musculus albino
A. Mathur and A. Saxena
Department of Zoology
Raj Rishi College, Alwar (Rajasthan)
The efficacy of
anticoagulants, Brodifacoum and Difethialone on poison shy
albino mice ( with a prior feeding of sub lethal dose of zinc
phosphide ) reveal a markedly high suspectibility in no choice
bait feeding with a low range of days (2-4 with 0.005% conc.
Brodifacoum and 3-7 days with 0.000625 % conc. Difethialone).
The acute median lethal dose, LD50 for poison shy albino mice
also showed an increase oral toxicity after an prior ingestion
of zinc phosphide.
Key words :
Anticoagulants/Brodifacoum/Difethialone/Poison-shy population,
Oral Toxicity.
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6. |
Asian J. Exp. Sci,
Vol. 13, No. 1&2, 1999, 27-35
Significance of
Serum Copper, Zinc and Copper/Zinc Ratio in Cancer Head and Neck
and Cervix Uterus Patients Treated with Radiotherapy
A. Chougle and S. Hussain**
*Department of Radiotherapy
RNT Medical College, Udaipur - 313 001
**Meera Girls College, Udaipur
The Trace elements
such as copper (Cu) and Zinc (Zn) are very essential for normal
physiological activities of human being. Inadequate levels of
these trace elements may cause disfunctioning or illness. We
have estimated serum copper levels (SCL) and serum zinc levels (SZL)
in 70 patients with head and neck malignancy and 57 patients
with malignancy of cervix uterus. The estimations were done on
atomic absorption spectrometer [902/903 GBC] in blood samples
collected before start of radiotherapy, midway between
radiotherapy (30 Gy tumor dose), at completion of therapy (60-70
Gy tumor dose) and during subsequent monthly follow-ups.
Estimation were also done in control group of 20 healthy
individuals and were 109.5 ± 18.02 µg/100ml, 84.05 ± 12.3 µg/100
ml and 1.27 ± 0.30 for SCL, SZL and Cu/Zn ratio respectively. In
the present study we observed significantly higher levels of
copper in both head and neck and cervix malignancies . In head
and neck the copper levels before start of radiotherapy were
234.1 ± 28.1, 257.2 ± 24.8, 281.2 ± 25.6 (µg/100ml) for stage
IIA, IIB and IIA respectively. Similarly the levels in cancer
cervix were 263.8 ± 13.0, 305.5 ± 11.5, 312.2 ± 54.7
(µg/100ml)for stage II, IIIA and IIB respectively . The SZL were
lower than the control group in both malignancies of head and
neck and cervix. We further found that the SCL and Cu/Zn ratio
to decrease as tumor regresses where as SZL levels were observed
to increase. The Cu/Zn ratio can be used to predict the
prognosis of the disease to radiotherapy in head and neck and
cervix malignancies.
Key words:
Copper, Zinc, Malignancy, Copper/Zinc, Tumor regression,
Biochemical investigation
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7. |
Asian J. Exp. Sci,
Vol. 13, No. 1&2, 1999, 37-46
Management of Malignancies by
Radiotherapy and Role of Biochemical Parameters
A. Chougule,
S. Hussain* and D.P. Singh**
Department of Radiotherapy
SMS Medical College and Hospital,
Jaipur-302 004
*Meera Girls College,
Udaipur-313 001
**RNT Medical College,
Udaipur-313 001
There is fast
accumulating evidence that environmental factors play a very
significant role in occurrence of malignancy. The environmental
factors alter cellular growth leading to biochemical changes in
blood serum. In the present communication we have made an
attempt to evaluate the biochemical changes such as macrometals
(magnesium and calcium), serum pseudocholine esterase (PCHE) and
serum lactate de hydrogenase (LDH) levels as side adjuct in
diagnosis and prognosis of 126 biopsy proved malignancies of
head & neck and cervix of uterus.
In the present communication we observed that PCHE levels were
within normal limits in early stage of malignancy whereas they
were significantly lowered in advanced stage of malignancy. As
radiotherapy progressed the level again tend towards normal
limit. The LDH activity was much higher than the normal limits
in all the cases of malignancy. The LDH activity became normal
after completion of radiotherapy in all the patients with no
tumor activity. Hyper calcemia and hyper magnesia was observed
in advanced malignancy and level decreased as radiotherapy
progressed. From present study we conclude that the biochemical
tests in combination can be employed to know the extent of tumor
activity and has got excellent prognostic significance.
Key words:
Malignancy, Biochemical Markers, Hypercalcemia |
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